Modification of heat-induced whey protein isolate hydrogel with highly bioactive glass particles results in promising biomaterial for bone tissue engineering

This study deals with the design and comprehensive evaluation of novel hydrogels based on whey protein isolate (WPI) for tissue regeneration. So far, WPI has been considered mainly as a food industry by-product there are very few reports application in engineering (TE). In this work, WPI-based were modified bioactive glass (BG), which is commonly used bone substitute material. Ready-to-use, sterile produced by simple technique, namely heat-induced gelation. Two different concentrations (10 20% w/w) sol–gel-derived BG particles two sizes (2.5 <45 µm) compared. µCT analysis showed that highly porous almost 100% pore interconnectivity. generally homogenously distributed hydrogel matrix, affecting size, reducing material porosity. Thermal presence matrix reduced water content improved their thermal stability. decreased enzymatic degradation materials. The materials underwent mineralization simulated biological fluids (PBS SBF) possessed high radical scavenging capacity. vitro tests indicated cytocompatible supported MG-63 osteoblastic cell functions.